| 张翼飞,张月超,张建强,王东,王银平,范中伟,刘剑.复杂地质条件下生物炭强化微生物降解氯代烃污染地下水研究及应用[J].地质找矿论丛,2025,40(4):542-549 |
| 复杂地质条件下生物炭强化微生物降解氯代烃污染地下水研究及应用 |
| Study and Application of Biochar-Enhanced Microbial Degradation of Chlorinated Hydrocarbon Contaminated Groundwater under Complex Geological Conditions |
| 投稿时间：2025-11-26 修订日期：2025-12-03 |
| DOI：10.6053/j.issn.1001-1412.2025.04.011 |
| 中文关键词: 复杂地质条件 氯代烃 生物降解 生物炭 地下水 |
| 英文关键词:complex geological conditions chlorinated hydrocarbons biodegradation biochar groundwater |
| 基金项目:天津市重点研发计划院市合作项目“基于高效生物菌群智能构建的有机污染土壤低碳修复关键技术研发及应用示范”（25YFYSHZ00050） 资助。 |
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| 中文摘要: |
| 随着工业化进程的加速，有机氯代烃污染已成为全球地下水环境面临的严峻挑战之一，尤其在水文地质条件复杂的京津冀区域，传统生物修复技术往往难以达到理想效果。针对复杂地质条件下地下水污染生物修复效率低的问题，本研究在典型氯代烃污染场地筛选到一株降解菌，该菌为Stutzerimonas kunmingensis GF-1（简称GF-1降解菌），从GF-1降解菌的生长情况和对邻二氯苯的降解结果分析，该菌可以直接利用邻二氯苯，将其分解。本文同时研究以秸秆基生物炭作为载体，构造新型的生物炭复合菌剂材料，新型材料的降解实验结果表明，只有 GF-1降解菌存在的实验中，当初始浓度为 500 mg/L时，邻二氯苯的去除率为 70.48 %；在生物炭负载 GF-1降解菌的体系中，初始浓度为800 mg/L时，邻二氯苯的降解率仍维持在75.36 %。生物炭负载GF-1降解菌可以有效地强化生物降解效果。实际污染地块试验结果表明，经过强化生物修复33天后，氯苯、邻二氯苯、1，2-二氯乙烷、三氯乙烷和三氯乙烯均达到地下水质量标准Ⅳ类水平（GB/T14848-2017）。生物炭强化生物修复技术具有二次污染风险低，成本可控的优势，具有很好的工程应用前景。 |
| 英文摘要: |
| With the acceleration of the industrialization process, organic chlorinated hydrocarbon contamination has become one of the serious challenges facing the global groundwater environment, especially in the Beijing-Tianjin-Hebei region with complex hydrogeological conditions, where traditional bioremediation technologies often fail to achieve satisfactory results. This paper addresses the issue of low bioremediation efficiency of groundwater contamination under complex geological conditions. A degrading strain isolated from a typical chlorinated hydrocarbon-contaminated site was studied. The strain was identified as Stutzerimonas kunmingensis GF-1（named GF-1）. The strain’s growth and dichlorobenzene degradation results indicate that the strain can directly utilize dichlorobenzene and decompose it. This paper also studied the construction of a new type of biochar composite microbial agent material using straw-based biochar as a carrier. The degradation experimental results for the new material show that, when the initial concentration was 500 mg/L, and only the degrading strain was present, the removal rate of dichlorobenzene was 70.48 %. In the system with biochar carrying the degrading strain, at an initial concentration of 800 mg/L, the dichlorobenzene degradation rate remained at 75.36 %. Biochar carrying the degrading strain can effectively enhance biodegradation. The pilot experiment conducted at the actual contaminated site showed that after 33 days of enhanced bioremediation, chlorobenzene, dichlorobenzene, 1,2-dichloroethane, trichloroethane, and trichloroethylene all met the Class IV groundwater quality standard (GB/T14848-2017). The biochar-enhanced bioremediation technology has low secondary pollution risk, controllable costs, and good engineering application prospects. |
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